Enrollment took place from 2012 to 2015 with a total of 3,044 dogs enrolled. In order to enroll, dogs must have been between 6 months and 2 years of age, provide a two-generation pedigree, live in the contiguous United States, and be deemed free of life-threatening diseases by a veterinarian. Each enrolled dog had a baseline study visit, laboratory testing and had questionnaires completed by the owner and veterinarian.
